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STEMCELL Technologies Inc easysep d magnetic particles
Easysep D Magnetic Particles, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+d+magnetic+particles/easysep+human+t+cell+isolation+kit/pmc11010340-300-35-41
Average 90 stars, based on 1 article reviews

easysep d magnetic particles - by Bioz Stars, 2026-09

90/100 stars

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Isolation:

Article Title: The impact of hyperglycaemia on PKM2-mediated NLRP3 inflammasome/stress granule signalling in macrophages and its correlation with plaque vulnerability: an in vivo and in vitro study.
Article Snippet: a Department of Cardiology, Xuanwu Hospital, Capital Medical University, National Clinical Research Centre for Geriatric Diseases, Beijing 100053, China b Department of Occupational and Environmental Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang 110122, China c Surgical Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China d Beijing Fangshan District Liangxiang Hospital, Beijing 102501, China

other:

Article Title: Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33.
Article Snippet: 1Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France.. 2These authors contributed equally: Corinne Cayrol and Anais Duval.. *e-mail: corinne.cayrol@ipbs.fr; jean-philippe.girard@ipbs.fr Allergic diseases such as asthma, atopic dermatitis (eczema), allergic rhinitis (hay fever) and allergic conjunctivitis are common diseases that affect patients from childhood to old age1,2.

Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
Article Snippet: In brief, Lin − cells were enriched by two successive rounds of depletion of Lin + cells using biotin-conjugated antibodies to CD5, CD11b, CD19, CD45R/B220, Ly-6G/C (Gr-1), Ter119, and 7-4 and Easysep D magnetic particles (Mouse Hematopoietic progenitor cell enrichment kit, Stem cell Technologies).

Article Title: Simultaneous, single particle, magnetization and size measurements of micron sized, magnetic particles
Article Snippet: Settling velocity vs. magnetic velocity – show the results of experimental analysis of EasySep TM D, Dynal M280 and M450 particles evaluated with the electromagnetic, CTV system (0.2A; B = 0.103 T; S m = 8.4 × 10 TA/m 2 )., and are dot plots of settling versus magnetic velocity (mm/s), whereas 3B, 3C, 4B, 4C, and 5B, 5C are histograms of settling and magnetic velocities, respectively. fig ft0 fig mode=article f1 caption a4 Dot plot, 3A, and histograms of the settling velocity and magnetic velocity, 3B and 3C, respectively, of EasySep D magnetic particles (Stem Cell Technologies Inc. 19250) using the electromagnet CTV (0.2A; S m = 0.84 TA/mm 3 ). fig ft0 fig mode=article f1 Figure 4 caption a4 Dot plot,4A, and histograms of the settling velocity and magnetic velocity, 4B and 4C, respectively, of Dynal M280 magnetic particles using the electromagnet CTV (0.2A; S m = 0.84 TA/mm 3 ). fig ft0 fig mode=article f1 caption a4 Dot plot, 5A, and histograms of the settling velocity and magnetic velocity, 5B and 5C, respectively, of the Dynal M450 particles in PBS solution using the electromagnet CTV (0.2A; S m = 0.84 TA/mm 3 ).

Transferring:

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. Pipette tips 15 ml centrifuge tubes (Fisherbrand, catalog number: 07–200-886) 50 ml centrifuge tubes (Fisherbrand, catalog number: 0553913) Tissue culture plates, 24-well transparent (Falcon, catalog number: 353047) White, 96-well plates, sterile with white lids (PerkinElmer, catalog number: 6005181) EDTA tubes purple tops for blood collection: Vacutainer brand sterile (BD, catalog number: 366643) CryoTubeTM vials for freezing cells (Thermo Fischer Scientific, catalog numbers: 153779, 202209, 363401) Tissue culture plates, 48-well transparent (Falcon, catalog number: 353078) 5 ml round-bottom polystyrene tubes (Corning, Falcon ® , catalog number: 352054) TZM-bl cells (NIH AIDS Reagent program, catalog number: 8129) Animal serum complex (Gemini Bio Products, Fetal PlexTM, catalog number: 100–602), storage in a −18 °C freezer before opening bottle and then store at 4 °C for subsequent use DMSO (Life Technologies Corp, catalog number: 20688) FBS (HyClone, catalog number: SH3011803) Beta-Glo Assay System (Promega, catalog number: E4780), storage in a −18 °C freezer Custom ordered resting CD4+ T cells negative selection kit (Stemcell Technologies, catalog number: 19309VK) with EasySep D Magnetic Particles (Stemcell Technologies, catalog number: 19250), storage at 4 °C DynabeadsTM Human T-Activator CD3/CD28 (Thermo Fischer Scientific, GibcoTM, catalog number: 11131D) Clinical grade Human Recombinant Interleukin-2: IL-2 Powder 22 MU VIAL (65483011607), storage in a −18 °C freezer where MU stands for medical units Note: Alternatively, human recombinant IL-2 can be purchased from Life Technologies Corp, catalog number: PHC-0023. .. Human Recombinant Interleukin-7: IL-7 Premium grade (Miltenyi Biotec, catalog number: 130–095-361), storage in a −18 °C freezer Efaviranz (EFV) (Cayman Chemical, catalog number: 14412), need to be re-suspended in IMDM media and diluted according to need of the experimental procedure Flow Monoclonal Antibodies Note: You will use 5 μl of each antibody for staining.

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. Materials and Reagents list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Pipette tips 15 ml centrifuge tubes (Fisherbrand, catalog number: 07–200-886) 50 ml centrifuge tubes (Fisherbrand, catalog number: 0553913) Tissue culture plates, 24-well transparent (Falcon, catalog number: 353047) White, 96-well plates, sterile with white lids (PerkinElmer, catalog number: 6005181) EDTA tubes purple tops for blood collection: Vacutainer brand sterile (BD, catalog number: 366643) CryoTubeTM vials for freezing cells (Thermo Fischer Scientific, catalog numbers: 153779, 202209, 363401) Tissue culture plates, 48-well transparent (Falcon, catalog number: 353078) 5 ml round-bottom polystyrene tubes (Corning, Falcon ® , catalog number: 352054) TZM-bl cells (NIH AIDS Reagent program, catalog number: 8129) Animal serum complex (Gemini Bio Products, Fetal PlexTM, catalog number: 100–602), storage in a −18 °C freezer before opening bottle and then store at 4 °C for subsequent use DMSO (Life Technologies Corp, catalog number: 20688) FBS (HyClone, catalog number: SH3011803) Beta-Glo Assay System (Promega, catalog number: E4780), storage in a −18 °C freezer Custom ordered resting CD4+ T cells negative selection kit (Stemcell Technologies, catalog number: 19309VK) with EasySep D Magnetic Particles (Stemcell Technologies, catalog number: 19250), storage at 4 °C DynabeadsTM Human T-Activator CD3/CD28 (Thermo Fischer Scientific, GibcoTM, catalog number: 11131D) Clinical grade Human Recombinant Interleukin-2: IL-2 Powder 22 MU VIAL (65483011607), storage in a −18 °C freezer where MU stands for medical units Note: Alternatively, human recombinant IL-2 can be purchased from Life Technologies Corp, catalog number: PHC-0023. .. Human Recombinant Interleukin-7: IL-7 Premium grade (Miltenyi Biotec, catalog number: 130–095-361), storage in a −18 °C freezer Efaviranz (EFV) (Cayman Chemical, catalog number: 14412), need to be re-suspended in IMDM media and diluted according to need of the experimental procedure Flow Monoclonal Antibodies Note: You will use 5 μl of each antibody for staining.

Sterility:

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. Materials and Reagents list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Pipette tips 15 ml centrifuge tubes (Fisherbrand, catalog number: 07–200-886) 50 ml centrifuge tubes (Fisherbrand, catalog number: 0553913) Tissue culture plates, 24-well transparent (Falcon, catalog number: 353047) White, 96-well plates, sterile with white lids (PerkinElmer, catalog number: 6005181) EDTA tubes purple tops for blood collection: Vacutainer brand sterile (BD, catalog number: 366643) CryoTubeTM vials for freezing cells (Thermo Fischer Scientific, catalog numbers: 153779, 202209, 363401) Tissue culture plates, 48-well transparent (Falcon, catalog number: 353078) 5 ml round-bottom polystyrene tubes (Corning, Falcon ® , catalog number: 352054) TZM-bl cells (NIH AIDS Reagent program, catalog number: 8129) Animal serum complex (Gemini Bio Products, Fetal PlexTM, catalog number: 100–602), storage in a −18 °C freezer before opening bottle and then store at 4 °C for subsequent use DMSO (Life Technologies Corp, catalog number: 20688) FBS (HyClone, catalog number: SH3011803) Beta-Glo Assay System (Promega, catalog number: E4780), storage in a −18 °C freezer Custom ordered resting CD4+ T cells negative selection kit (Stemcell Technologies, catalog number: 19309VK) with EasySep D Magnetic Particles (Stemcell Technologies, catalog number: 19250), storage at 4 °C DynabeadsTM Human T-Activator CD3/CD28 (Thermo Fischer Scientific, GibcoTM, catalog number: 11131D) Clinical grade Human Recombinant Interleukin-2: IL-2 Powder 22 MU VIAL (65483011607), storage in a −18 °C freezer where MU stands for medical units Note: Alternatively, human recombinant IL-2 can be purchased from Life Technologies Corp, catalog number: PHC-0023. .. Human Recombinant Interleukin-7: IL-7 Premium grade (Miltenyi Biotec, catalog number: 130–095-361), storage in a −18 °C freezer Efaviranz (EFV) (Cayman Chemical, catalog number: 14412), need to be re-suspended in IMDM media and diluted according to need of the experimental procedure Flow Monoclonal Antibodies Note: You will use 5 μl of each antibody for staining.

Selection:

Article Title: A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins.
Article Snippet: Lentiviral (LV) vectors have emerged as powerful tools for basic research and clinical applications because of their ability to stably transduce both dividing and nondividing cells.. A wide range of viral envelope (Env) glycoproteins have the ability to associate with the membrane of LV vectors, a process that is referred to as pseudotyping.. Pseudotyped vectors have the capacity to transduce specific cell types for specific applications. .. Lentiviral (LV) vectors have emerged as powerful tools for basic research and clinical applications because of their ability to stably transduce both dividing and nondividing cells.. A wide range of viral envelope (Env) glycoproteins have the ability to associate with the membrane of LV vectors, a process that is referred to as pseudotyping.. Pseudotyped vectors have the capacity to transduce specific cell types for specific applications. .. Lentiviral (LV) vectors have emerged as powerful tools for basic research and clinical applications because of their ability to stably transduce both dividing and nondividing cells.. A wide range of viral envelope (Env) glycoproteins have the ability to associate with the membrane of LV vectors, a process that is referred to as pseudotyping.. Pseudotyped vectors have the capacity to transduce specific cell types for specific applications.

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. Materials and Reagents list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Pipette tips 15 ml centrifuge tubes (Fisherbrand, catalog number: 07–200-886) 50 ml centrifuge tubes (Fisherbrand, catalog number: 0553913) Tissue culture plates, 24-well transparent (Falcon, catalog number: 353047) White, 96-well plates, sterile with white lids (PerkinElmer, catalog number: 6005181) EDTA tubes purple tops for blood collection: Vacutainer brand sterile (BD, catalog number: 366643) CryoTubeTM vials for freezing cells (Thermo Fischer Scientific, catalog numbers: 153779, 202209, 363401) Tissue culture plates, 48-well transparent (Falcon, catalog number: 353078) 5 ml round-bottom polystyrene tubes (Corning, Falcon ® , catalog number: 352054) TZM-bl cells (NIH AIDS Reagent program, catalog number: 8129) Animal serum complex (Gemini Bio Products, Fetal PlexTM, catalog number: 100–602), storage in a −18 °C freezer before opening bottle and then store at 4 °C for subsequent use DMSO (Life Technologies Corp, catalog number: 20688) FBS (HyClone, catalog number: SH3011803) Beta-Glo Assay System (Promega, catalog number: E4780), storage in a −18 °C freezer Custom ordered resting CD4+ T cells negative selection kit (Stemcell Technologies, catalog number: 19309VK) with EasySep D Magnetic Particles (Stemcell Technologies, catalog number: 19250), storage at 4 °C DynabeadsTM Human T-Activator CD3/CD28 (Thermo Fischer Scientific, GibcoTM, catalog number: 11131D) Clinical grade Human Recombinant Interleukin-2: IL-2 Powder 22 MU VIAL (65483011607), storage in a −18 °C freezer where MU stands for medical units Note: Alternatively, human recombinant IL-2 can be purchased from Life Technologies Corp, catalog number: PHC-0023. .. Human Recombinant Interleukin-7: IL-7 Premium grade (Miltenyi Biotec, catalog number: 130–095-361), storage in a −18 °C freezer Efaviranz (EFV) (Cayman Chemical, catalog number: 14412), need to be re-suspended in IMDM media and diluted according to need of the experimental procedure Flow Monoclonal Antibodies Note: You will use 5 μl of each antibody for staining.

Recombinant:

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. Materials and Reagents list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Pipette tips 15 ml centrifuge tubes (Fisherbrand, catalog number: 07–200-886) 50 ml centrifuge tubes (Fisherbrand, catalog number: 0553913) Tissue culture plates, 24-well transparent (Falcon, catalog number: 353047) White, 96-well plates, sterile with white lids (PerkinElmer, catalog number: 6005181) EDTA tubes purple tops for blood collection: Vacutainer brand sterile (BD, catalog number: 366643) CryoTubeTM vials for freezing cells (Thermo Fischer Scientific, catalog numbers: 153779, 202209, 363401) Tissue culture plates, 48-well transparent (Falcon, catalog number: 353078) 5 ml round-bottom polystyrene tubes (Corning, Falcon ® , catalog number: 352054) TZM-bl cells (NIH AIDS Reagent program, catalog number: 8129) Animal serum complex (Gemini Bio Products, Fetal PlexTM, catalog number: 100–602), storage in a −18 °C freezer before opening bottle and then store at 4 °C for subsequent use DMSO (Life Technologies Corp, catalog number: 20688) FBS (HyClone, catalog number: SH3011803) Beta-Glo Assay System (Promega, catalog number: E4780), storage in a −18 °C freezer Custom ordered resting CD4+ T cells negative selection kit (Stemcell Technologies, catalog number: 19309VK) with EasySep D Magnetic Particles (Stemcell Technologies, catalog number: 19250), storage at 4 °C DynabeadsTM Human T-Activator CD3/CD28 (Thermo Fischer Scientific, GibcoTM, catalog number: 11131D) Clinical grade Human Recombinant Interleukin-2: IL-2 Powder 22 MU VIAL (65483011607), storage in a −18 °C freezer where MU stands for medical units Note: Alternatively, human recombinant IL-2 can be purchased from Life Technologies Corp, catalog number: PHC-0023. .. Human Recombinant Interleukin-7: IL-7 Premium grade (Miltenyi Biotec, catalog number: 130–095-361), storage in a −18 °C freezer Efaviranz (EFV) (Cayman Chemical, catalog number: 14412), need to be re-suspended in IMDM media and diluted according to need of the experimental procedure Flow Monoclonal Antibodies Note: You will use 5 μl of each antibody for staining.

Incubation:

Article Title: Effect of novel proteasome and immunoproteasome inhibitors on dendritic cell maturation, function, and expression of IκB and NFκB.
Article Snippet: Contents lists available at ScienceDirect Transplant Immunology j ourna l homepage: www.e lsev ie r .com/ locate / t r im Effect of novel proteasome and immunoproteasome inhibitors on dendritic cell maturation, function, and expression of IκB and NFκB Ahmad Samer Al-Homsi a,⁎, Zhongbin Lai b,c, Tara S. Roy a, Nicholas Kouttab c a Division of Adult Blood and Marrow Transplantation, Spectrum Health, Michigan State College of Human Medicine, Grand Rapids, MI, USA b Division of Hematological Malignancies and Blood and Marrow Transplantation, Roger Williams Medical Center, Providence, RI, USA c Department of Immunopathology, Roger Williams Medical Center, Providence, RI, USA ⁎ Corresponding author at: Division of Adult Blood and 406, 221 Michigan Street NE, Grand Rapids, MI, 49503, fax: +1 616 486 6489.. E-mail address: Ahmad.Al-Homsi@SpectrumHealth.or 0966-3274/$ – see front matter © 2013 Published by Else http://dx.doi.org/10.1016/j.trim.2013.09.011 a b s t r a c t a r t i c l e i n f o Article history: Received 21 August 2013 Received in revised form 24 September 2013 Accepted 27 September 2013 Keywords: Proteasome inhibitor Immunoproteasome inhibitor Dendritic cell GvHD Dendritic cells (DC) play a central role in the pathophysiology of graft versus host disease (GvHD).. Their antigen presenting capacity is nuclear factor κB- (NF-κB) dependent.

Review